anti cd54 apc (Miltenyi Biotec)
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anti cd54 apc
Anti Cd54 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd54+apc/CD54+(ICAM-1)+Antibody%2C+anti-human%2C+REAfinity/pm41864987-137-11-16
Average 94 stars, based on 35 article reviews
Anti Cd54 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd54+apc/CD54+(ICAM-1)+Antibody%2C+anti-human%2C+REAfinity/pm41864987-137-11-16
Average 94 stars, based on 35 article reviews
anti cd54 apc - by Bioz Stars,
2026-10
94/100 stars
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Staining:Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation. Article Snippet: .. For staining the cells were incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi, #130-113- 444), REA Control (S)-PE (Miltenyi, #130-113-438), REA Control (S)-APC (Miltenyi, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi, #130-113-440); Article Title: The Monocytic Cell Line THP-1 as a Validated and Robust Surrogate Model for Human Dendritic Cells Article Snippet: .. Cells were stained with the following antibodies: diluted 1:50, REA Control (S)-VioGreen (Miltenyi Biotec, #130-113-444), REA Control (S)-PE (Miltenyi Biotec, #130-113-438), REA Control (S)-APC (Miltenyi Biotec, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi Biotec, #130-113-440); HLA-DR-VioGreen (Miltenyi Biotec, #130-111-795), Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation Article Snippet: .. For staining the cells were incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi, #130-113-444), REA Control (S)-PE (Miltenyi, #130-113-438), REA Control (S)-APC (Miltenyi, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi, #130-113-440); Article Title: Extensive monolayer formation depends on a subpopulation of transplanted human iPSC-derived RPE cells Article Snippet: .. P1 cells were stained with Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization Article Snippet: After 24 h, cells were harvested, washed three times in autoMACS running buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany), and transferred to 96-well plates (2 × 10 5 cells per antibody panel). .. Cells were stained with the following antibodies (diluted 1:50) for 10 min at 4 °C in the dark: REA Control (S)-APC (Miltenyi Biotec, #130-113-434), Incubation:Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation. Article Snippet: .. For staining the cells were incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi, #130-113- 444), REA Control (S)-PE (Miltenyi, #130-113-438), REA Control (S)-APC (Miltenyi, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi, #130-113-440); Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation Article Snippet: .. For staining the cells were incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi, #130-113-444), REA Control (S)-PE (Miltenyi, #130-113-438), REA Control (S)-APC (Miltenyi, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi, #130-113-440); Article Title: Incorporating immune cell surrogates into a full-thickness tissue equivalent of human skin to characterize dendritic cell activation. Article Snippet: Surface marker detection via flow cytometry Cells were harvested after differentiation or treatment and washed in autoMacs Running Buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany). .. At least 1 × 105 cells for each antibody panel were transferred to 96-well u-bottom plates and incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi Biotec, #130-113-444), REA Control (S)-PE (Miltenyi Biotec, #130-113-438), REA Control (S)-APC (Miltenyi Biotec, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi Biotec, #130- 113-440); HLA-DR-VioGreen (Miltenyi Biotec, #130-111-948), CD1a-PE (Miltenyi Biotec, #130-112-022); CD207-PE-Vio770 (Miltenyi Biotec, #130-112-370), Control:Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation. Article Snippet: .. For staining the cells were incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi, #130-113- 444), REA Control (S)-PE (Miltenyi, #130-113-438), REA Control (S)-APC (Miltenyi, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi, #130-113-440); Article Title: The Monocytic Cell Line THP-1 as a Validated and Robust Surrogate Model for Human Dendritic Cells Article Snippet: .. Cells were stained with the following antibodies: diluted 1:50, REA Control (S)-VioGreen (Miltenyi Biotec, #130-113-444), REA Control (S)-PE (Miltenyi Biotec, #130-113-438), REA Control (S)-APC (Miltenyi Biotec, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi Biotec, #130-113-440); HLA-DR-VioGreen (Miltenyi Biotec, #130-111-795), Article Title: A human 3D immune competent full-thickness skin model mimicking dermal dendritic cell activation Article Snippet: .. For staining the cells were incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi, #130-113-444), REA Control (S)-PE (Miltenyi, #130-113-438), REA Control (S)-APC (Miltenyi, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi, #130-113-440); Article Title: Incorporating immune cell surrogates into a full-thickness tissue equivalent of human skin to characterize dendritic cell activation. Article Snippet: Surface marker detection via flow cytometry Cells were harvested after differentiation or treatment and washed in autoMacs Running Buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany). .. At least 1 × 105 cells for each antibody panel were transferred to 96-well u-bottom plates and incubated in Automacs Running Buffer with the following antibodies (1:50): REA Control (S)-VioGreen (Miltenyi Biotec, #130-113-444), REA Control (S)-PE (Miltenyi Biotec, #130-113-438), REA Control (S)-APC (Miltenyi Biotec, #130-113-434); REA Control (S)-PE-Vio770, (Miltenyi Biotec, #130- 113-440); HLA-DR-VioGreen (Miltenyi Biotec, #130-111-948), CD1a-PE (Miltenyi Biotec, #130-112-022); CD207-PE-Vio770 (Miltenyi Biotec, #130-112-370), Article Title: Pseudopterosin A-D Modulates Dendritic Cell Activation in Skin Sensitization Article Snippet: After 24 h, cells were harvested, washed three times in autoMACS running buffer (Miltenyi Biotec, #130-091-221, Gladbach, Germany), and transferred to 96-well plates (2 × 10 5 cells per antibody panel). .. Cells were stained with the following antibodies (diluted 1:50) for 10 min at 4 °C in the dark: REA Control (S)-APC (Miltenyi Biotec, #130-113-434), Blocking Assay:Article Title: Multiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides. Article Snippet: Nickel Sulphate (NiSO4, Sigma-Aldrich, 656895), 2,4-dinitrochlorobenzene (DNCB; Sigma-Aldrich, 237329), and lactic acid (LA, Sigma-Aldrich, PHR1215) were purchased from Sigma-Aldrich and selected based upon the criteria detailed in the general guidance from Organisation for Economic Cooperation and Development (OECD). .. The fluorophore-conjugated antibodies CD86-PE and |
![Pseudoperosin (Ps)A-D reduces the nickel sulfate (NiSO 4 ) induced upregulation of DC activation markers <t>CD54</t> and CD86 in a similar manner as dexamethasone. THP-1-derived dermal dendritic cell surrogates (DDCs) were seeded with 1 × 10 6 cells/mL into a 24-well plate. Cells were pre-treated with dexamethasone [1 µM] or PsA-D [20 µM] for 1 h, followed by NiSO 4 [380 µM] treatment for 23 h. Surface marker analysis was performed via flow cytometry and is depicted as histograms ( A ) and percentage of positive cells ( B ). Error bars indicate the standard errors of the mean ( n = 3 independent experiments with * = p ≤ 0.05 and ** = p ≤ 0.01).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3997/pmc12193997/pmc12193997__marinedrugs-23-00245-g002.jpg)
